Spectroscopic techniques is one of the physico-chemical analysis of the observed interactions of at

Spectroscopic techniques is one of the physico-chemical analysis of the observed interactions of atoms or molecules with electromagnetic radiation (REM). REM interaction with the molecule will produce one or two kinds of three events that may occur as a result of the interaction of molecules with REM atoms are scattering (scattering), absorption (absorbtion) and emission (emission) by REM atoms or molecules are observed.
In spectroscopic techniques, there are two kinds of instruments are spectrometers and spectrophotometers. Spectrometer using a monochromator quarterly slit fixed at the focal plane, while the spectrometer spectrophotometer is equipped with a detector that is electrically photos.
In the pharmaceutical field, the analysis of UV-Vis Spectrophotometer mrnggunakan quarterly known as the primary method, both for the identification, examination and for the assay purity. Absorption spectrophotometry is the measurement of the absorption of certain wavelengths of electromagnetic radiation are narrow, monochromatic quarterly approach, the absorbed substances. Uptake measurements can be performed in the ultraviolet region (190-380 nm wavelength) or in the visible region (380-780 nm wavelength).
A molecule will absorb energy from the outside when the amount of energy equal to the energy needed by the molecules to make the transition in energy levels, quarterly therefore different levels of each molecule the energy required by the compound is not the same, ie, a certain amount in accordance with the formula:
c = concentration (g / L)
Beer's law deviations can be caused due to the ideal experimental conditions are not met, namely: Monochromatic light is not enough. The light side (stay radiation) of the detector. Change the detector sensitivity. The intensity of the light source and detector amplifiers vary due to unstable voltage. At equilibrium dissociation chemical change, such as the change in pH of the solution. Fluorescent solution. Solution temperature change during the measurement.
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