Sunday, December 21, 2014

Stool samples should then be delivered to the laboratory as soon as possible to be taken on the sel


In third world countries, about the deaths of children due to diarrhea and dehydration is followed. In developed countries, especially in healthy adults are also seen in the order that they are suffering from debilitating diarrhea. The family nuevosoi Enterobacteriaceae, the genus Salmonella and Shigella are the causes nuevosoi of bacterial diarrhea, although the two genera in the family nuevosoi classified as common organ affected, but the properties of microbiological and epidemiological differences are large.
Shigella infections is primarily a disease of children and 70% of these infections occur in children under 15 years. Endemic diseases among adults and children in contact with the family occurs, the outbreak in child care centers, boarding houses and maintains nuevosoi individual institutions nuevosoi can be seen, Shigella infection through fecal - oral, especially in those are transmitted by contaminated hands. Since Shigella bacteria can cause disease in fewer than 200 Shigella infections quickly in communities where health standards are low and the level of personal hygiene should be promoted.
By severe abdominal cramps and passing the disease is painful and frequent stools containing blood and mucus is characterized by low volume, symptoms one to three days after the entry of bacteria into the body occurs there neutrophils, erythrocyte feces and mucus plenty Shigella is a sign of pollution.
In 1896, the Shiga bacillus that causes an epidemic of dysentery in Japan discovered in the definitive diagnosis of amoebic dysentery and Basylr nuevosoi possible nuevosoi Pzyrshd, four years after the Flexner dysentery several cases examined in the Philippines and the Mykrrvby similar to Shiga bacillus was reported. Shigella dysenteriae is the most common cause of dysentery in the early part of this century, but since then Sony and flexneri nuevosoi strains in most countries are responsible for most cases.
Serogroups A, B, and C serogroup D samples are similar but vary in terms of biochemical, all species of Shigella dysentery Bassilly but can cause severe illness, death of occurrence of each species is different.
Sony is able to ferment lactose Shigella flexneri and Shigella serotypes lungs can produce gas from glucose, these microorganisms can survive in water at room temperature for up to six months, Shigella is therefore sensitive to acid in stool samples nuevosoi to be sent to laboratories Prompt be cultured in differentiation medium. According to the mannitol fermentation into two separate strains of serogroup D, C and B group consisting of mannitol mannitol positive and negative, including the most important serotypes of serogroup A first group s.dysenteriae 1 is called Shigella bacillus strains, although this A rare cause of severe diarrhea Bassilly types is important.
Temperature and relative humidity are factors that lead to the survival of Shigella darkness in public toilets are different Shigella in water for at least 15 days in seawater live and survive in the right circumstances, milk and other can easily food to survive.
Flexneri intestinal bacillus spores produce so little heat and concentrations of disinfectants and regular Shvnd.fnl deadly organisms are easily damaged, formaldehyde, glutaraldehyde and beta-halogen compounds are killing bacteria. The quaternary ammonium compounds according to the particular shape or adding chlorine to water is effective in controlling the spread of disease, lack of bowel.
For the isolation of Shigella in the best way possible to be both selective medium (selective) used a different environment with the choice of versatile low as Garmk Cancan (MAC) and a more selective agar agar as Gzylvzlyzyn Dzksy chelate (XLD) other environments where moderate to high levels of choice and can be a good alternative for XLD agar are Dzksy citrate chelate (DCA) and Hktvn enteric agar (HE), the agar Salmonella - Shigella (SS) should not be used because it often Shigella dysenteriae serotypes can be a barrier to growth.
Stool samples should then be delivered to the laboratory as soon as possible to be taken on the selective plates can be inoculated with just a drop of liquid or suspension of the stool, other way is to cottonswabs anal or cottonswabs the stool should be used. If the swab is used to inoculate selective medium, the space of approximately 2.5 cm in diameter on the surface of agar plates containing impregnated and then the plate is drawn to the separation line (streak) plates have a period of 18 Up to 24 hours at 35 to 37 C after heating rate and the growth rate in each separate area used for each patient nuevosoi samples are recorded. Shigella colonies on (MAC) as colorless colonies convex, about 2 to 3 mm in diameter appear.
Colonies Shigella dysenteriae 1 (sd1) may be smaller than this. Shigella colonies on XLD agar colonies are smooth as one to two millimeters in diameter, pink or red translucent colonies on agar XLD sd1 unlike other Shigella species are often very small. Suspected colonies from the MAC and XLD plates were selected and screened as KIA or TSI is inoculated into the environment.
To respond that the KIA or TSI done any actual biochemical tests is necessary for inoculation of pure cultures are used, at least one of each of the separate colonies of each bridge

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