Blood agar blood agar medium general media is to reproduce and isolate pathogenic bacteria, especia

NAC DESOXY xylose lysine medium agar (XLD) Xylose-lysin-decarboxycholate-agar
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Blood agar blood agar medium general media is to reproduce and isolate pathogenic bacteria, especially bacteria of the nutrients they need for growth, the environment hemolysin Rvd.blavh used in bacteria can also be explored. After autoclaving the base when the temperature reached 50 C, defibrinated bovine blood at a rate of 5 to 7 percent, and added to the medium under sterile conditions into sterile petri plates. Depending on the type of bacteria is one of the three conditions below. 1. Complete hemolysis β: Bacterial colonies containing enzymes hemolysin and around the transparent area is created. 2. incomplete hemolysis α: lysis of bacteria containing enzymes hemolysin but less than 50% and a green halo around the colony is created. 3. The absence of hemolysis γ: bacteria lacking the enzyme is hemolysin Shklath Chocolate agar agar medium if the blood agar base medium after autoclaving broth when the temperature is around 80-70 C, defibrinated bovine blood is added, resulting Shklath agar medium comes. In this environment, because the red blood cells by heating dismantled and its components removed, the growth of bacteria such as Haemophilus and Neisseria that more needs to be prepared food is good. Blood agar agar medium such as blood or hemolysis hemolysis incomplete depending on the type of bacteria is causing the lack of hemolysis. Brilliant Green Agar is a selective medium for the isolation environment Briliant green agar is used for Salmonella broth spp. The environment of the growth of intestinal bacteria (Enterobacteriaceae) except Salmonella prevents. If Ecoli, Klebsiella, Proteus and Escherichia Baktryas h to grow, because ferment lactose or sucrose, or both, and form acids, in the presence of phenol red to yellow in color reagent Come In If you are lactose-negative bacteria such as Salmonella grow, due to lack of reagent in the presence of glucose fermentation medium comes in purple. Mac Cancan Macconkey agar agar medium selective medium for the isolation and identification of gram-negative bacteria is. Bile salts and crystal violet inhibits the growth of Gram-positive bacteria are. Bacteria ferment the lactose (lactose-positive) Klnyhayy purple and Baktryhayyy that are able to ferment (lactose negative) colorless Klnyhayy develop. Purple broth colonies of lactose-positive broth bacteria of the acid reaction is the result of fermentation of lactose in the presence of bile and Neutral red uptake was obtained. Neutral red color represents an alkaline environment and is red in acidic environments. Sobor dextrose agar medium Saborad Dextrose agar selective medium for the propagation of fungi, broth which grow many bacteria in the environment. The selectivity broth of the medium for fungi based on low PH and the food is poor. DNASE bacteria culture medium containing D-oxy Raybv nuclease enzyme, a pink halo around the colonies create. Toluidine blue is blue in the presence of intact DNA. But if normal shedding HCL destroys the DNA molecule and the compound comes in pink. The main use of Mueller broth Hinton broth, Mueller hinton agar medium for susceptibility testing of bacteria (antibiogram) method which hard disk Several bacteria broth such as Neisseria meningitides N.gonorrhoeae growth are Fastisious it grow. Fletcher medium Fleche's medium for the isolation and maintenance of Leptospira used in this environment. After sterilization and cooling of the medium, to which was added 80 mL of sterile rabbit serum and then poured into the 7-5 ml sterile tubes for 30 minutes at a temperature of 56 C, we are to complement in serum is inactive. For the isolation of Leptospira, blood-urine and tissue samples suspected in the medium and the incubation broth temperature of 29 C for 30 days or more. Alternatively, the cultivation spread Vbvsylh prepared microscope with dark-field control of our culture. Lptvspraha proliferation of small opacities in the environment pose a milky appearance of a loop at the top of the can suggest is Lptvspra growth. Methyl red medium - Vzhvsprvskar Methylred-Vegesproskaure broth (MR-VP) this is the test criterion for the separation of glucose fermentation pathways and ultimately produce organic acids or Bvtyln Bvtandyvl glycol which of these tests detect broth coliforms in the case of is used. Also, the distinction between S. and micrococcus species from the environment is used. Testing MR: After incubation at 37 C for 48 h cultivation, value added 6.0 ml of methyl red color represents the color red and MR test is positive. The MR appearance broth of orange test is weakly positive and negative MR in case of change and stability testing is yellow. The result: the emergence of a positive test --------- red (PH = 4/4) appearance of orange ------- weak positive test (PH = 5/3) yellow color ---- ----- negative (PH = 5/3) preparation of reagents: MR 1-methyl red hot 2-ethanol 04/0 -------- -------- 40 mL of 3 - | -------- distilled water and 100 ml of methyl red in ethanol solution to bring the volume to 100 ml. Testing VP: the culture broth tubes and incubated for about 6/0 ml of reagent broth alpha Nftvl (reagent A) and 2.0 mL of the KOH solution (unexposed